histological and histomorphometrical study of endocrine pancreas in maquee sheep

this study was conducted to determine the properties of islets of langerhans in pancreas of adult and immature, male and female maquee sheep. for this purpose, 20 samples of pancreas were used equally for each male and female, immature and adult animals. paraffin sections that they were stained by h&e; method were used for mean distribution and dimension of langerhans islets, and maldonado staining method were performed for defining of a and b cells. all data that were obtained from histomorphometrical study were analyzed by anova and bonferroni statistic process by spss software. in this study a cells determined by red granules and b cells with blue granules in their cytoplasm. morphometrical study of langerhans islets showed that mean distribution of islets in different lobes (right, left, and body) of pancreas were not significantly different in any studied groups. mean dimension of langerhans islets were significantly different only between the right lobe of immature male and adult female(p<0.05). additionally, mean distribution of a and b cells in different groups were significantly different (p<0.05). there were not significant differences in mean distribution of total cell count of langerhans islets in any groups. this study revealed that, the highest mean distribution of langerhans islets were in adult males, and least of them were in adult female sheep. the mean dimension of langerhans islets in immature males were significantly higher than the other groups (p<0.05).